Composite

Part:BBa_K3117047

Designed by: Lena Schorr   Group: iGEM19_FAU_Erlangen   (2019-10-18)


scFv against GPA33 with SpyCatcher codon optimized for E.coli

Part BBa_K3117047 is a fusion protein of an anti-GPA33 single-chain variable fragment (scFv) and a SpyCatcher.


Usage and Biology

By connecting the variable regions of the heavy and light chain of an anti-GPA33 antibody with a short, flexible GGGGS linker (BBa_K3117028), the scFv retains it's antigen-binding ability and is much smaller than a conventional antibody. The SpyCatcher attached to the scFv belongs to the SpyTag/SpyCatcher system, of which the sequence is derived of the FbaB protein in the bacterium Streptococcus pyogenes. Once it comes into contact with its corresponding other part, the SpyTag (BBa_K3117015), they bind covalently (Hatlem et al., 2019).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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